LC-MS/MS data of bioengineering modification of the glycome using Salmonella (Ying Sheng)

We established a metabolic-engineered host cell model that generates controlled glycan structure phenotypes on the cell surface. Specifically, we produced host cell surfaces that were primarily fucosylated, sialylated, undecorated complex type structures, and high mannose-type structures. The manipulated host cell N-glycomes were characterized and confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Furthermore, glycoproteomic analysis yielded site-specific N-glycosylation information. Glycopeptides were identified and quantified using a standard glycoproteomic workflow. Collectively, our results suggested the importance of glycans in host-microbe interactions and provided a novel insight into the significance of host glycome during pathogenesis.