GNPS_HMP_cultures_full_set

Extracts of HMP project isolates that were grown successfully. Full dataset.
The information on the correspondence of MS data to taxonomy is listed here:
https://docs.google.com/spreadsheets/d/1ZaZbwiQS086rDyi_rqzEykz5BFLDMT3YcI0kV7Z9aYA/edit?usp=sharing
The document is currently being updated.
Sample preparation procedure:
The supernatant collected from cell cultures was processed to make it compatible with HLPC-MS analysis. The solid phase extraction with wash was carried out to reduce impact of cell culture media which is highly detrimental for the ESI. The 30 mg sorbent Oasis HLB (Waters, Waltham, MA) SPE cartridges were used to achieve broad metabolite coverage. The cell samples were stored at -80? and thawed at room temperature immediately prior to extraction. The thawed samples were then centrifuged for 10 minutes at 1200 rpm and extracted. For the SPE extraction, the Oasis HLB SPE cartridge was conditioned with 700µL of 100% HPLC grade methanol and equilibrated with 700µL of HPLC grade DI water. The cell supernatant (~350-400µL) was loaded into cartridge and allowed to slowly elute. The loaded SPE wells were then washed with 800µL of 5% methanol in water and the absorbed material was slowly eluted with 200 µL of 100% methanol. Vacuum up to ~ 20 psi was applied for the wells that did not elute within an hour. The collected eluent was stored at -20? until the HPLC-MS analysis.