Transcriptomic analysis of sulfur dioxide stress-resistant Saccharomyces cerevisiae strain obtained by evolutionary engineering

In this study, we have developed a highly SO2-stress-resistant yeast (Saccharomyces cerevisiae) strain [F3] using evolutionary engineering, by successive batch selection at gradually increased SO2 levels. The evolved F3 strain was resistant to 1.0 mM SO2 stress, which was strongly inhibitory to the reference strain. Whole-transcriptomic analysis of F3 was performed with respect to its reference strain to determine differences in gene expression levels between the two strains. Saccharomyces cerevisiae Agilent Yeast One-Color DNA microarray systems were used for comparative whole-genome transcriptomic analysis of the selected mutant F3 and its reference strain. Prior to gene expression analysis, each culture was grown in triplicate in yeast minimal medium (YMM) at 30°C and 150 rpm. Total RNA isolation was carried out, using RNeasy Mini Kit (Qiagen) at early exponential phase of growth (OD600 ~1.0).