Transcriptomic analysis of cellular senescence induced by ectopic expression of ATF6a in human breast cancer cells

To investigate the molecular mechanism that triggers endoplasmic reticulum (ER) stress-induced cellular senescence in breast cancer cells, we established MCF7 cell lines exposed to ATF6a ectopic expression. We then performed gene expression profiling analysis using data obtained from RNA-seq of 9 different cells at three time points. Overall design: Comparative gene expression profiling analysis of RNA-seq data for MCF-7 cells from three distinct time points (baseline, 6 hours, and 9 hours after ATF6a ectopic expression), using Illumina HiSeq X10 and NovaSeq 6000 platforms with paired-end 100 base sequencing.