Analysis of hnRNP K interacting RNAs in myeloid KG-1a cells

HNRNPK is part of the minimally deleted region (MDR) in AML del(9q). To understand the function of hnRNP K in pathogenesis of this AML subtype, we analyzed the function of hnRNP K as an RNA binding protein in myeloid cells. Co-immunoprecipitated RNAs from hnRNP K and non-related control immunoprecipitation as well as input RNA were subjected to next generation sequencing. This analysis revealed an interaction of hnRNP K with 1076 RNAs, among them many mRNAs encoding transcription factors involved in myeloid differentiation and AML pathogenesis. Cytoplasmic extracts from KG-1a cells were subjected to immunopreciptiation with a hnRNP K specific antibody or a non-related control reaction. Total RNA from cytoplasmic extract was isolated as input. Three independent biological replicates were analyzed.