Characterization of the Microbiome in the Infant Diapered Area Skin

This is the study to characterize the infant diaper area skin microbiome. Microbial swabs were taken at the genitals, intertriginous (leg folds), perianal region and buttocks after the regions were graded for rash by a trained grader. Rash grading was completed using a validated grader scoring tool. The tool categorizes Diaper Rash on a 7-point scale, assessing skin redness, degree of skin coverage, and presence of papules and/or pustules. 16S/ITS 454 Pyrosequencing was used to sequence the V1-V3 region of bacterial 16S gene and the ITS region of fungal rRNA gene.