BS-seq of ros1-3, ros1-4 with wildtype col-0

To servey the methylome changes by mutation of a DNA demethylase ROS1, we performed the Next-gen sequencing (NGS) associated whole genome bisulfite sequencing (BS-seq) analysis. Two ROS1 knockout lines (ros1-3 and ros1-4; Penterman et al. 2007 [PMID: 17409185]) were subjected in this study. Overall design: Whole genome bisulfite sequencing of ros1-3 and ros1-4 with the wildtype col-0 plants at 12-d-old, using Illumina HiSeq 2000 system