Neutralization of Genotype 1 Rabies strains determined by Rapid Fluorescent Focus Inhibition Test (RFFIT) with cell grown-virus.

aMean IC50 (nM) of triplicates.bTissue Culture Infectious Dose 50%, which corresponds with the dilution of the infected brain suspension – VHH mixture which yields 50% infection of neuroblastoma cells of isolates: 9912CBG (dog, Cambodia), 9147 FRA (fox France), CVS (strain IP13), 9722 POL (raccoon dog, Poland), 8740 THA (Human, Thailand), 070591C (dog, Ivory coast), 9009 NIG (dog, Niger). Neutralization considered if a minimum of 100-fold reduction of virus infectivity in the brain was observed after preincubation with antibody (Mab 8-2) or VHH compared to a control VHH (NR4 or NR1 anti-RSV) (#neutralization/#total).