JLehrstrand/3D_imaging_of_an_entire_T1D_pancreas

---
license: cc-by-nc-4.0
language:
- en
tags:
- biology
---
Title: 3D imaging of an entire pancreas shows inverse proportions of extra islet vs islet associated β cells in late onset type 1 diabetes

Abstract: Residual β-cell function can positively affect diabetes regulation in type 1 diabetes (T1D), but details on residual β-cell mass distribution in T1D is largely lacking in a whole organ context. Implementing an optical 3D imaging pipeline, we generated a complete account of the remaining β-cells throughout an entire human late onset T1D pancreas at a microscopic resolution. Our data show that the majority of the residual β-cells were present as scattered individual cells or as punctated clusters of individual β-cells, spatially separated from each other and all other endocrine cell-types. Compared to islet-associated β-cells, extra-islet β-cells appeared in a significantly higher relative abundance in the head region of the T1D pancreas. This 3D depiction of an entire T1D pancreas shows that individual β-cells may be preserved and/or formed in a highly regionalized manner, potentially reflecting key aspects of disease dynamics, advocating for increased focus on extra-islet islet β-cells in attempts to develop strategies for pancreatic β-cell preservation in T1D.

Extracted data: Includes raw and compiled datapoints underlying Fig. 1E, F-H, 3M, L, 4C, S2A-D, S4E-F, S6A-E and S9A.

"LSFM" and "OPT" directories contain raw image data in *.tif format to allow reproduction of quantitative assessments.