LEP system, IL-6, and TNF-α mRNA expression in different tissues of primiparous cows supplemented with or without CLA in a time course of 105 DIM.

CLA: Lutrell® Pure, BASF SE, Ludwigshafen, Germany.Control: Silafat®, BASF SE.Significant differences between different days per tissue are defined using different letters. Significant differences (P≤0.05) between CLA and control group within day and tissue are depicted by bold numbers.Data are normalized based on the geometric mean of Eukariotic translation initiation factor 3 (EIF3K), Lipoprotein receptor-related protein 10 (LRP10), RNA polymerase II (POLR2A), Emerin (EMD), Marvel domain containing 1 (MARVELD1), and Hippocalcin-like 1 (HPCAL1) for each s.c. fat and mesenteric fat depots; EIF3K, LRP10, POLR2A, EMD, and MARVELD1 for omental and retroperitoneal fat depots; HPCAL1, LRP10, POLR2A, EIF3K, Glyceraldehyde-phosphate-dehydrogenase (GAPDH) for liver; LRP10, EMD, POLR2A, EIF3K for muscle, and MARVELD1, EMD, LRP10, EIF3K, POLR2A, HPCAL1 for mammary gland tissue.1 Leptin. 2 Leptin receptor isoform b. 3 Tumor necrosis factor-α. 4 Interleukin-6; Means ± SE.