RGEN-SEQ FOR AMPLIFICATION-FREE SCREEN OF OFF-TARGET SITES OF GENE EDITORS
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https://www.ncbi.nlm.nih.gov/sra/SRP326574
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资源简介：
We describe a sensitive, PCR-free next-generation sequencing method (RGEN-seq) for unbiased detection of double-stranded breaks generated by RNA-guided CRISPR-Cas9 endonuclease. The method is extremely simple, and it is on a par or even supersedes in sensitivity other assays while not relying on amplification steps.
创建时间：
2022-07-31
