Tetramethylammonium Chloride and Dimethyl Sulfoxide improve SARS-CoV-2 detection in individual/pooled saliva samples with RT-qPCR

Supplementary Material 1. Excel file with 4 spreadsheets, organized as mentioned: The first two tabs compare the nucleotide sequences of primers and probes for the N1 and N2 regions used in the CDC Kit for SARS-CoV-2 identification. These sequences correspond to consensus sequences of the Alpha, Beta, Delta, Gamma, GH490R, Lambda, Mu, and Omicron variants of SARS-CoV-2, generated with 20% and 100% thresholds, as published in Cadena-Caballero et al. 2022 (https://doi.org/10.12688/f1000research.109673.1). The number of possible substitutions per position is also provided. In addition, it is indicated the percentage identity between probes and primers targeting the consensus sequences of the aforementioned variants with 20% and 100% thresholds. The third and fourth tabs present RT-qPCR results for single and multiplexed saliva samples, respectively.