Effects of the glucagon-like 1 receptor agonist exendin-4 on activated gut intraepithelial lymphocytes

Gut intraepithelial lymphocytes (IELs) are one of the few immune cell populations in the body that expresses glucagon-like 1 receptors (GLP-1R). To test the potential effects of GLP-1 on gut IEL function, we performed bulk RNA-seq on gut IELs isolated from C57BL/6J mice treated with anti-CD3 and with or without exendin-4 for 3 hours. 4 anti-CD3- and PBS-treated and 4 anti-CD3- and exendin-4-treated C57BL/6J mice. Total RNA isolated from sorted gut IELs was prepared into stranded mRNA library using TruSeq stranded mRNA library preparation kit (Illumina) and sequenced on a NovaSeq (Illumina) with a read length of 2 x 200 bp.