Gene expression in human endometrium cells (ATCC 4003) [control]
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https://www.ncbi.nlm.nih.gov/sra/SRP251381
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To detect the gene profiles in non-decidualized stromal cells, cultured stromal cells were collected and subjected to RNA-Seq. After aligned to mouse mm10 by HISAT2, RPKM value was calculated by Edger. Our results show that SOX4 is one of the most abundant gene in the whole SOX family. The expression of SOX4 was further confirmed by qPCR, ISH and immunofluorescence. The expression of SOX4 in human endometrium indicated the essential role of SOX4 for reproduction. This RNA-Seq data provides fundamental information for our further physiological study of SOX4. Overall design: Total RNAs in whole uterus from cultured 4003 stromal cells were extracted by TRIzol and purified by ploy-A before subjected to RNA-Seq by HiSeq2500. The expression of level of each gene is normalized to RPKM.
创建时间:
2020-03-07



