Selected suppressors of anchorless proliferation.

A selection of genes involved in suppression of anchorless proliferation is listed. The complete list is found in S2 Table. The absolute number of transforming vectors identified per suppressor transcript by screening both LEGO-TBX2 and LEGO-p53 is shown as well as the percentage these vectors constitute of the maximum number of vectors that could enzymatically be produced from each AluI site (see also Figure I in S1 Fig). Suppressor transcripts were ranked according to the score obtained by multiplying the absolute number of identified transforming vectors and the percentage of transforming shRNA vectors produced per transcript. In addition, its induction in DKO RASV12 MEFs after loss of anchoring, and its repression in anchorless DKO RASV12 TBX2 or DKO RASV12 p53kd cells compared to anchorless DKO RASV12 cells are shown. Genes reduced >1.66 fold in anchorless DKO RASV12 TBX2 or DKO RASV12 p53kd cells are shown in bold, genes induced >1.66 fold in DKO RASV12 after loss of anchoring are shown in italics.