Observed heterozygosity is related to position of the locus in the genome.

FIS is the inbreeding coefficient that ranges from −1 (fixed heterozygosity) to +1 (complete heterozygote deficit). Position refers to the location within a supercontig in Assembly 1 (September 7, 2006) of the first variable position of the locus. Supercontigs are ordered in descending size from 1.1 (2.38 Mbp) to 1.69 (0.56 Mbp). The test of HWE is the probability that data are drawn from a population in Hardy Weinberg equilibrium, calculated using an exact test [84] as implemented in Genepop v. 3.4 [81]. The test of HWE was conducted for both uncorrected and clone-corrected data sets. Significant values of the test are P<0.05.