Fission yeast telomeres are packaged into a non-canonical histone-containing chromatin structure dependent on Shelterin and RNA.

Yeast telomeres exist in a distinct protected structure at the chromsome end. This structure, termed the telosome, has long been thought to be free of histones. Here we show that this is not the case - telomere repeats are indeed associated with histones. This finding has implications for telomere function and ultrastructure. Overall design: ChIP-Seq analysis of H3K9Me2 in Schizosaccharomyces pombe. 2 biological replicates of 1 L wild-type cells grown in YE4S to OD600=0.6 cells used per replicate, crosslinked in formaldehyde. ChIP carried out with antibodies to H3K9Me2, input and IP DNA purified was purified and processed for sequencing.