Cloning and Bioinformatics Analysis of vscB Gene of T3SS Chaperone of Vibrio alginolyticus

[Objectives] To clone and analyze the vscB gene of Vibrio alginolyticus HY9901 by bioinformatics. [Methods] A pair of specific primers were designed according to the vscB gene sequence of Vibrio alginolyticus HY9901. The full length of the primers was cloned by PCR and analyzed by bioinformatics. [Results] The vscB gene was 429 bp long, encoding 142 amino acids, with a theoretical molecular weight of 16.4 kDa and a pI value of 5.48. Amino acid sequence analysis of VscB showed that VscB was not a secretory protein, without signal peptide and transmembrane region, and there were protein kinase C phosphorylation site and casein kinase II phosphorylation site in the sequence. Homologous comparison of amino acid sequences showed that VscB of V. alginolyticus had the highest protein similarity with Vibrio Parahaemolyticus, reaching 91%. Phylogenetic tree analysis showed that the corresponding proteins of V. alginolyticus VscB, Vibrio Parahaemolyticus and Vibrio diabolicus were clustered in the same subfamily. Functional domain analysis showed that it had CesT family domain. Tertiary structure prediction showed that there were 3 α-helices and 5 β-turns in VscB protein. [Conclusions] This study provided a theoretical basis for further study on the function of chaperone of V. alginolyticus.