The Hxk1 protein of Candida albicans influences gene expression of both metabolic and virulence related genes

The Hxk1 protein of Candida albicans is a moonlighting protein with a wide variety of functions. It phosphorylates N-acetylglucosamine (GlcNAc) which is necessary for different purposes in the cell including energy and chitin production. Further, this protein is a regulator for morphogenesis independent of its function during GlcNAc catabolism.To identify the regulatory role of Hxk1 and putative downstream regulator Hxk2, we performed CUT&RUN experiments for GFP-tagged Hxk1 strains and GFP-tagged Hxk2 strains. CUT&RUN for 3 biological replicates of GFP-tagged Hxk1, GFP-tagged Hxk2 and corresponding IgG negative controls.