DREAM-seq, an endonucleases-assisted mapping of R-loops

Genome-wide identification of R-loops almost uniquely relies on antibody based pull-down, a method that shows sequence and structural biases. Here we describe a novel approach – DREAM-seq – centered around the use of endonuclease digestions. Unstimulated and stimulated B cells showed that R-loops are more widespread than anticipated, mostly covering all actively transcribed genes. Overall design: 6 stimulated B cell samples, including 3 Mock and 3 RNaseH treated samples. 4 unstimulated B cell samples, with 2 Mock and 2 RNaseH treated samples.