Genetic map distances and distribution of parental and recombinant progeny in <i>msh4</i>, <i>msh5</i> mutants in the NHY942/NHY943 strain background.

All mutants are isogenic derivatives of NHY942/NHY943 (Materials and Methods). For single spores, recombination frequencies (recombinant spores/total spores) were multiplied by 100 to yield genetic map distances (cM). 95% confidence intervals for genetic map distance in the single spores were determined using VassarStats (http://faculty.vassar.edu/lowry/VassarStats.html). For tetrads, genetic distance in centimorgans (cM) was calculated using the RANA software without considering aberrant segregants [24]. The Stahl Laboratory Online Tools website (http://groik.com/stahl/) was used to calculate standard error around the genetic distance for tetrads. n; number of single spores, N; four spore viable tetrads analyzed; Par, parental single spores; Rec, recombinant single spores; S.E; standard error. Wild-type, pch2Δ, spo11-HA and pch2Δ spo11-HA data are from Zanders and Alani [21].