RNA Sequencing Analysis of Pam3CSK4-stimulated BMDMs with or without Dopamine (DA)

Mice Pam3CSK4-stimulated bone marrow derived macrophages with DA or not genome.Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the most prominent downregulated pathway upon DA treatment was NF-kappaB signaling pathway. Consistently, the heatmap analysis displayed significantly decreased expression in many genes associated with NF-kappaB signaling and inflammation, such as Il6, Tnf, Il12b, Il1b, and Ccl5 Pam3CSK4-stimulated BMDMs with or without DA mRNA profiles were generated by deep sequencing, in triplicate, using BGISEQ-500