Induction of E. coli BL21(DE3) with various IPTG concentrations and incubation temperatures
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Cultivation of Escherichia coli BL21(DE3) expressing L-Asparginase type 2 from E. coli inserted in a plasmid pET28a. Cultivation at 23°C (Experiment group R2006), 30°C (Experiment group R2005), or 37°C (Experiment group R2004). Induction with IPTG at initial time at concentration: 0, 20, 60, 100, or 1000 µmol/L.
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Zenodo创建时间：
2025-02-27
