Demonstration data underlying the article: Wide Field Light-sheet Microscopy with Lens-axicon Controlled Two-photon Bessel Beam Illumination

<b>Summary</b><br> This data record provides details of the data supporting the claims of the article: “Wide Field Light-sheet Microscopy with Lens-axicon Controlled Two-photon Bessel Beam Illumination”, which proposes simple illumination optics, a lens-axicon triplet composed of an axicon and two convex lenses, to generate longer extent Bessel beams, towards broadening applications of two-photon excitation in light-sheet microscopy. Type of data: custom-built two-photon excitation digital scanned light-sheet microscope possessing the beam full width at half maximum of 600–1000 μm with less than a 4-μm waist when using 10× illumination lens and ~2-3-μm axial resolution Subject of data: cellular dynamics over the whole body of medaka fish <b>Data</b> The data underlying the related article are contained in the zipped folder ‘DataSubmit.zip’, which is openly available as part of this <i>figshare </i>data record. The zipped folder contains a hierarchical sub-folder structure, which is listed briefly below. Further details on the images in each sub-folder are provided in the Word file ‘DataInfo.docx’. <b>1. FLT4angiography (related to Fig.5A)</b> This folder includes subfolders numbered 1-3. These are biologically independent samples. Each subfolder includes unsigned 16bit tiff-image sequences labeled as Image_z****_c***.tif (z:z-stack number, c:channel number; c001:GFP, c002:Rhodamine). Sizes of images are 1. 1029×4419px 1130 z-seq 2. 1030×3774px 969 z-seq 3. 1030×3812px 530 z-seq <b>2. FLT4development (related to Fig.7)</b><br> This folder includes subfolders numbered 1 and 2. These are biologically independent samples. Each subfolder includes unsigned 8bit z-stack tiff images labeled as t****min.tif. The numbers denote time (minites post fertilization; 1. 4755-9305min, 2. 4890-9220min), and the time-lapse interval is 5min. Sizes of images are 1. 512×512px 350 z-seq 2. 512×512px 360 z-seq <b>3. Caimaging (related to Fig.8)</b><br> This folder includes unsigned 16bit tiff images labeled as image_t***_z***.tif. (t: time slice number, z: z-stack number). The time-lapse interval is 4sec. Size of images: 512×512px 67 z-seq <br> <b>Corresponding author(s) for this study</b> Takashi Saitou, Department of Molecular Medicine for Pathogenesis, Graduate School of Medicine, Ehime University, Shitsukawa, Toon, 791-0295, Ehime, Japan. E-mail: [e-mail]. Tel.: [phone]. Fax.: [phone]. <br> <b>Ethics</b> All experiments were conducted in accordance with the guidelines of the ethics committee for animal experiments of Ehime University.