TABLE 1 in Phylogeny of North America's largest cicada radiation redefines Tibicinoides and Okanagana (Hemiptera: Auchenorrhyncha: Cicadidae: Tibicininae)

TABLE 1. PCR primers and reaction conditions. GenePrimerPrimer sequenceAnnealing timeExtension conditionsReferenceEF1αEF1a-F650-ambigTGCTGCKGGTACTGGTGAAT15 s68℃, 75 sMarshall et al. 2018EF1-N-1419ACACCAGTTTCAACTCTGCCSueur et al. 2007ARD1ARD1-1041FTGGAAAGTGTTTCTGTCACATTTTCG15 s68℃, 75 sMarshall et al. 2018ARD1-1733RATCTCTTTTCATAGCGTATGCGTCARD1ForShortCGCTTTGTGAGAGAATTRCARD1RevShortGTATGCGTCTTCACCRTCTGCCAMCal-60-forAACGAAGTAGATGCCGATGG45 s72℃, 150 sBuckley et al. 2006Cal-72-revGTGTCCTTCATTTTNCKTGCCATCATUBC Insect Primer16SLR-J-12887CCGGTCTGAACTCAGATCACGT15 s68℃, 75 sSimon et al. 1994LR-N-13398CGCCTGTTTAACAAAAACATCOIJerryTGACAACAYYTATTTTGATTTTTTGG60 s72℃, 75 sSanborn et al. 2021PatTGATTCATTGCACTAATCTGCCATATTAJerryHUCAACATTTGTTCTGATTCTTTGGThis studyPatHUTTCATTGCACTATTCTGCCATATTACOIITL2-J-3033AATATGGCAGATTAGTGC60 s72℃, 75 sSimon et al. 1994TGACOIIATGCTATATCTCCTATAATAGACCThis studyTK-N-3786GTTTAAGAGACCATTACTTSimon et al. 1994