Identification of competing endogenous RNA (ceRNA) and micro-RNA profiles and regulatory networks in 4-nonylphenol-induced impairment of sertoli cells I

Purpose: To uncover the mechanism of 4-nonylphenol-induced impairment of sertoli cells, we used RNA sequencing technology to compare the lncRNA expression differences between the control groups and the 4-nonylphenol treated groups. Sertoli cells lncRNA, circRNA, mRNA profiles of the control groups and the 4-nonylphenol treated groups were generated by deep sequencing, in duplicate. The library quality was determined using a Bioanalyzer 2100 (Agilent). The Illumina hiseq X ten platform was used for lnc RNA sequencing. The quality of RNA-seq reads was examined using FastQC. 4-nonylphenol-treated and control sertoli cells lncRNA, circRNA, mRNA profiles of 24h were generated by deep sequencing, in duplicate.