Signal integration and adaptive sensory diversity tuning in Escherichia coli chemotaxis

In uncertain environments, phenotypic diversity can be advantageous for survival. However, as the environmental uncertainty decreases, the relative advantage of having diverse phenotypes decreases. Here, we show how populations of E. coli integrate multiple chemical signals to adjust sensory diversity in response to changes in the prevalence of each ligand in the environment. Measuring kinase activity in single cells, we quantified the sensitivity distribution to various chemoattractants in different mixtures of background stimuli. We found that when ligands bind uncompetitively, the population tunes sensory diversity to each signal independently, decreasing diversity when the signal ambient concentration increases. However, amongst competitive ligands, the population can only decrease sensory diversity one ligand at a time. Mathematical modeling suggests that sensory diversity tuning benefits E. coli populations by modulating how many cells are committed to tracking each signal proport..., In-vivo single-cell FRET microscropy
Bacteria were grown to mid-exponential phase with inducer to express a YFP/RFP FRET pair. FRET imaging was performed with an inverted microscope (Eclipse Ti-E, Nikon) equipped with an oil immersion objective (CFI Apo TIRF 60X Oil, Nikon). Yellow fluorescent proteins were illuminated with a light-emitting diode system (SOLA SE, Lumencore) through one excitation filter (59026x, Chroma), then another (FF01-550/24-25, Semrock) and a dichroic mirror (F01-542/27-25F, Semrock). Emission was fed into an emission image splitter (OptoSplit II, Cairn) where it was split into donor and acceptor channels with a dichroic mirror (FF580-FDi01-25x36, Semrock) and collected through emission filters (FF520-Di02-25x36 and FF593-Di03-25x36, Semrock) with a scientific CMOS camera (ORCA-Flash 4.0 V2, Hammatsu). Red fluorescent protein mRFP1 was imaged in the same way as YFP, except with a different second excitation filter (FF01-575/05-25) and dichroic mirror (FF593-Di03-2..., , # Sensory diversity tuning in E. coli chemotaxis

[https://doi.org/10.5061/dryad.nvx0k6dzz](https://doi.org/10.5061/dryad.nvx0k6dzz)

**Signal Integration and Adaptive Sensory Diversity Tuning in *Escherichia coli* Chemotaxis**
Jeremy Philippe Moore, Keita Kamino, Rafaela Kottou, Thomas S. Shimizu, Thierry Emonet

**Dataset contents:** This dataset contains single-cell FRET time series measuring activity of the chemotaxis signaling pathway of E. coli.

## Description of the data and file structure

The dataset is a collection of .mat files which each contain a matlab struct with processed single-cell FRET data from different experiments. The structure of each .mat file is the same, but the experimental conditions are different. A list of experimental conditions (constant background ligand and foreground ligand whose concentration changes during the experiment) is provided in the file BackgroundList.txt.

Opening one of the .mat files in matlab will load the struct 'reorgData'. This stru...