Lin28b augments protein synthesis to control B cell positive selection early in life [RIP-seq]

In this study, we identify elevated protein synthesis as a defining characteristic of early life B cell development and a determinant for the qualitative switch in B cell output whereby self-reactive CD5+ B-1 cells becomes censored in postnatal mice This is in part mediated by the heterochronic RNA binding protein LIN28B. To identify Lin28b-dependent, stage specific effects, B cell progenitors at different developmental stages were sorted and analyzed by RNA-seq Overall design: B cell progenitors from whole bone marrow was expanded for five days under saturating concentration of IL-7 (20 ng/mL) before subjected to immunoprecipitation and poly-A targeted sequencing of co-IP RNA