Single-cell RNA-seq of E13.5 posterior palate from Foxd1 null mutant vs wt control

Cleft palate is a common major birth defect, of which surgical repair often fail to fully restore the structure and function of palatal muscles, leaving many patients suffering velopharyngeal dysfunction and/or impairment of speech and hearing. Foxd1 is enriched in the lateral mesenchyme of posterior palatal shelves, and _Foxd1_ null mutant mice display defects in posterior soft palate. To investigate the role of Foxd1 during soft palate development, single cell RNA-seq analysis using E13.5 posterior palatal tissue was performed using _Foxd1_ null mutant mice comparing with wildtype control embryos.