Validation of mouse model: qPCR and Western blots probing for Tor1a-torsinA

Model validation dataset associated with Pocratsky et al 2023, Science Translational Medicine

Data generated from postnatal day 18 littermate control (Cdx2::wt;Tor1a-wt/frt) and spinal Tor1a d-cko mice (Cdx2::FlpO;Tor1a-frt/frt) that develop early onset generalised torsional dystonia. Whole tissues were snap frozen and processed for expression of Tor1a and torsinA protein. Raw images of Western blots provided

Validation_qPCR.xlsx - dataset: Tor1a expression in brains and lumbar spinal cords

Validation_Westerns.xlsx - dataset: torsinA expression in brains and lumbar spinal cords

Western_metadata: details for attached images of blots

Full methodological detail provided in manuscript

与Pocratsky等人在2023年发表在《科学转化医学》上的研究相关的模型验证数据集。该数据集由产后第18天出生的同窝对照小鼠（Cdx2::wt;Tor1a-wt/frt）和脊髓Tor1a d-cko小鼠（Cdx2::FlpO;Tor1a-frt/frt）生成，这些小鼠早期出现全身性扭转性肌张力障碍。整个组织进行快速冷冻并处理以检测Tor1a和torsinA蛋白的表达。提供了Western印迹的原始图像。

验证_qPCR.xlsx - 数据集：大脑和腰椎脊髓中的Tor1a表达

验证_Westerns.xlsx - 数据集：大脑和腰椎脊髓中的torsinA表达

Western_metadata：附图印迹的详细信息

详细的方法论细节在论文中提供。