Human embryonic stem cell, chimpanzee induced pluripotent stem cell, orangutan induced pluripotent stem cell, rhesus embryonic stem cell, and their derived cortical organoid RNA-seq

We compared a 5 week time course of cortical organoid differentiation across human, chimpanzee, orangutan, and rhesus using bulk RNAseq. In addition, single cell RNAseq was performed on a subset of time points from human cells in weeks 0, 1, 2, and 5. Overall design: Bulk RNAseq of hESCs, ch-iPSCs, or-iPSCs, rhESCs, and each of 6 time points corresponding to weeks 0, 1, 2, 3, 4, and 5 during cortical organoid differentiation. Single cell RNAseq of human ESCs, week 1, week 2, and week 5 organoids.