Relative phzA2 transcript levels in the M18 and M18ΔRA strains during the exponential and stationary phases as assessed by qRT-PCR.

aValues were determined during the exponential phase (OD600 between 1.5 and 2.0) and the stationary phase (OD600 between 5.0 and 5.5). Values are means ± standard deviations of three independent experiments.bΔCT = mean CT,phzA2– mean CT,rpoD.c2–ΔΔCT, Normalised amount of cDNA from the phzA2 gene in the mutant M18ΔRA relative to that in the wild-type strain M18; ΔΔCT = mean ΔCT – meanΔCT,M18.