Real-time quantitative PCR analysis of 30 apple cyclophilin genes under abiotic stress

Plant cyclophilins are widely involved in a variety of abiotic stress regulation processes. Thirty cyclophilin members were identified from apple and their patterns of response to abiotic stress were examined. Apple seedlings with consistent growth were selected, cultivated under drought stress conditions simulated by PEG6000, sampled according to different time periods, and quickly frozen in liquid nitrogen.Total RNAs of apple plantlets were isolated using an RNA extraction kit (Tiangen, Beijing, China). qPCR gene expression profiling.Six samples were obtained from apple seedlings in different time periods (0h, 1h, 3h, 6h, 12h and 24h) after drought treatment.