RNA-seq of podocytes treated with glomerular endothelial cell vesicles

The effect of miRNA delivery from glomerular endothelial cells (GEnCs) to podocytes in vitro was examined by mRNA epression in podocytes untreated and treated with extracellular vesicles from GEnCs under varied activation conditions. Overall design: RNA sequencing of human podocytes untreated (UT) or treated with extracellular vesicles (EVs) derived from human glomerular endothelial cells (GEnCs) for 24 hours. EVs were isolated by ultracentrifugation from supernatants of GEnCs in a steady state (SS) or activated with glucose (Glu) or puromycin aminonucleoside (PAN) for 24 hours.