Blast Analysis of Monkeypox Virus Against Streptococcus pneumoniae Genomic Sequences

Introduction: The blastn analysis study was conducted between the sequences of Monkeypox viruses against Streptococcus pneumoniae H17. Considering there is no hitting when blast analysis with whole length of Streptococcus pneumoniae, we spliced the Monkeypox virus genome sequence into segments of 1,000bp with sliding windows of 50bp, and then conducted blast analysis on these segments against sequences of Streptococcus pneumoniae. Methods: The genome sequence of Monkeypox virus were spliced into segments of 1,000bp length, with starting positions shifting from 1 to 950 at interval of 50bp. In total 20 sets of segments were created. The 20 sets of Monkeypox genome segments were compared against sequences of Streptococcus pneumoniae downloaded from NCBI (https://ftp.ncbi.nlm.nih.gov/genomes/ all/GCF/002/076/835/GCF_002076835.1_ASM207683v1/GCF_002076835.1_ASM207683v1_genom ic.fna.gz) with blastn tools (2.13.0+) on a linux server, respectively. The hits/alignments on genome of Streptococcus pneumoniae reported by the blast analysis were retrieved with customized python scripts. Common hits across different sets of Monkeypox genome segments were merged. Results: There were 3449 hits reported by blast analysis between 3386 Monkeypox segments and 3370 Streptococcus pneumoniae sequences after merging all the blast results from 20 sets of Monkeypox genome segments. The dataset presented provides the results obtained.