Transcriptomics analysis of lineage-restricted Dendritic-cell (DC) progenitor cells in obesity
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https://www.ncbi.nlm.nih.gov/sra/SRP245437
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资源简介：
The overall goal of the project is to understand transcript-level changes in DC-progenitors from bone marrow of diet-induced obese mice by Next Generation Sequencing (NGS; RNASeq) Overall design: DC-restricted progenitors (Lin-/c-kit(int)/Sca1(lo)/CD115+/Flt3+; ~50,000 cells) from bone marrow (BM) compartment of high fat (60kcal % fat) & control chow (10 kcal%fat)-fed mic (n=4 per group) were flow-sorted and RNA was isolated. The whole transcriptome was amplified and library was constructed using NuGen Ovation Solo RNASeq System; and evaluated on high-sensitivity DNAchip (Agilent) and sequenced on a HiSeq 3000 platform
创建时间：
2020-03-24
