Single-cell RNA-sequencing of mouse colonic monocytes and macrophages (chow vs. high fat diet). Single-cell RNA-sequencing of mouse colonic monocytes and macrophages (chow vs. high fat diet)

We performed a single-cell transcriptome analysis of colon monocytes and macrophages in 2 mice fed with control diet (chow) and 2 mice fed with high-fat diet for 1 week. Overall design: CD11b+nonDCs colonic macrophages (liveCD45+Lin-CD11b+CD24- and pos for CD64 or Ly6C) were sorted for each replicate mouse, and used for single cell RNA-seq on a 10x genomics Chromium platform.