Transcriptome profiles of neonatal intestinal organoids under fibroblast coculture with or without LPS sitimulation and tryptophan metabolites intervention

We performed RNA sequencing to identify the role of fibroblast in the effects of tryptphan metabolites on LPS-stimulated mouse neonatal intestinal organoids. Overall design: The neonatal intestinal organoids were passaged and seeded into 24-well plates. Concurrently, fibroblasts were passaged and seeded into 0.4 µm pore-size transwell inserts, which were then placed into the wells containing organoids. The co-cultures were treated with lipopolysaccharide (LPS, 200 µg/mL), ILA or IAA (each at 6 µM), and/or AhR antagonist CH223191 (1 µM), based on the experimental conditions.