METHOD FOR PREPARING TRANSGENIC PLANT WITH INCREASED 20-HYDROXYECDYSONE CONTENT BY USING SPINACIA OLERACEA-DERIVED CYP85 GENE， AND PLANT PREPARED THEREBY

The present invention relates to a method for producing a transgenic plant which has increased content of 20-hydroxyecdysone compared to a wild type plant by transforming a plant cell with a recombinant vector containing a gene encoding CYP85 (cytochrome P450， 85 family) protein derived from spinach (Spinacia oleracea)， a transgenic plant with increased content of 20-hydroxyecdysone compared to a wild type plant which is produced by the above method and a seed thereof， a composition for increasing content of 20-hydroxyecdysone in plant which contains a gene encoding CYP85 protein derived from Spinacia oleracea as an effective component， a method for producing a transgenic plant with enhanced insect resistance according to transformation of a plant cell with a recombinant vector containing a gene encoding CYP85 derived from Spinacia oleracea， a transgenic plant with enhanced insect resistance which is produced by the above method and a seed thereof， and a composition for enhancing insect resistance of a plant which contains a gene encoding CYP85 protein derived from Spinacia oleracea as an effective component.