Inactivation of the nuclear interacting SET domain protein 1 (NSD1) impairs GATA1-regulated erythroid differentiation and causes erythroleukemia. NSD1 Add-back experiment, mRNASeq cultured erythroblasts.

In vitro differentiation of Nsd1-/- erythroblasts with retroviral expression of wildtype Nsd1, or a catalytically-inactive Nsd1N1918Q SET-domain Overall design: Add-back experiment with cultured Nsd1-/- erythroblasts rescued with either retroviral expression of wildtype Nsd1, or a catalytically-inactive Nsd1N1918Q SET-domain