Estimates of equilibrium concentrations and of model parameters derived from experiments performed in the cytoplasm.

Parameters derived from fits to FCS experiments performed using Bcd-EGFP during interphase in the cytoplasm (first column, using data from [15]) and values estimated for mitosis (second column) assuming that , and remained invariant and that during mitosis (diffusion coefficient estimate derived in. [15] using FRAP). The value was derived from fits to FCS experiments performed using NLS-EGFP. Two sets of FCS fits from [15] were used which gave the two limiting values listed in the Table (2 and 3 component fits, respectively). The mean fitting parameters reported in [15] were used to obtain the values listed in the table. All ratios listed are computed with respect to the total cytoplasmic Bcd concentration during interphase at the location where the FCS experiments were performed, .