Hypoxia differentially accelerates cognitive impairment related features in cerebral cortex revealed by single cell sequencing in APP/PS1 mice

Here we aimed at exploring the effect of hypoxia on cognitive impairment progression in a single cell resolution through single cell RNA sequencing (ScRNA seq). We exposed the APP/SP1 transgenic AD model mice to hypoxia condition and explored the cell heterogeneity and interactions in cerebral cortex with single cell RNA sequencing (10X genomics). We further analyze the differently expressed genes and signaling pathways related with cognitive impairment by different cell types and subclusters. Overall design: APP/PS1 mice was subjected to a hypoxia condition of 8% O2 for 5 hours. The cerebral cortex was then harvested and subjected to scRNA seq with 10X Genomics platform. Two samples of hypoxic or normoxic cortical tissue, respectively, were included in the analysis.