Heterozygous SNORA31 mutations in patients with herpes simplex encephalitis

Pseudo-seq was used to measure differences in the extent of pseudouridine (?) modification in rRNA from human cell lines with heterozygous, and homozygous disruptions of SNORA31. Overall design: CMC treatment of RNA followed by next generation sequencing was used to measure the extent of pseudouridylation at mapped human rRNA ? sites. CMC: carbodiimide N-cyclohexyl-N'-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate