Quantification of transcripts (RFE) by qPCR for genes not included in the figures. ^a

a, Data are presented as relative fold expression (RFE) and represent the changes in transcription compared to the bacteria grown in BHIN (value of 1.0). RNAs were isolated from EHEC O157:H7 strain 86-24 grown in BHIN, BHIN+bs, BHIN+lac, BHIN+bs+lac and MB in vitro and the bacteria recovered from loops inoculated with bacteria grown in BHIN (BHIN-Loop) and MB (MB-Loop) as well as the control loops inoculated with EMEM (Control-Loop). The levels of 16SrRNA transcripts were used to normalize the Ct values. Data are expressed as the means ± SD for RNA extracted in 4–6 replicates. *†, P<0.05 as compared to BHIN and to BHIN-Loop respectively.