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Identification of Nucleolin-binding targets and sequences in LPS-stimulated RAW 264.7 cells by PAR-CLIP

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NIAID Data Ecosystem2026-04-30 收录
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To investigate the consensus RNA-binding motifs of Nucleoli, we performed photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) analysis in nucleolar fractions from unstimulated or 12 h LPS-stimulated RAW 264.7 cells Overall design: We obtained NCL-bound RNAs from the nucleolar fractions of 12 h LPS-stimulated RAW264.7 cells and analyzed PAR-CLIP data using PARpipe analysis.

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2022-09-16
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