The effect of beta-glucan - induced trained immunity on the transcriptomic profile of bone marrow granulocyte-macrophage progenitors (GMP)

Induction of trained immunity by beta-glucan affects myeloid cells and their bone marrow progenitors. In particular, broad alterations in the transcriptome of trained myeloid cells have been demonstrated. In this study, we performed RNA sequencing in GMP from beta-glucan treated mice, as compared to control-treated mice, in order to investigate the impact of trained immunity on the transcriptomic profile of GMP. Overall design: WT mice were treated with a single dose of 1 mg of ß-glucan from Trametes versicolor (Invivogen) in PBS or with PBS vehicle alone (control) and after seven days bone marrow GMP (Lin—c-Kit+Sca1—CD16/32+CD34+) were sorted and RNA sequencing analysis was performed.