The age of the bone marrow microenvironment influences B-cell acute lymphoblastic leukemia progression via CXCR5-CXCL13 [RNA-Seq]

We performed RNA sequencing to determine the alteration in the molecular landscape in young versus old macrophages. Overall design: Young versus old BM macrophages were obtained by crushing femora, tibiae, and humeri of mice in phosphate-buffered saline (PBS) using mortar and pestle and then expanded in alpha-MEM medium supplemented with 20% fetal bovine serum, 1% penicillin/streptomycin and 1% L-glutamine, once non-adherent cells were removed. The immunophenotype of the adherent cells was confirmed by the following immunophenotype: F4/80+ CD169+.