Proteins present in scrapie associated fibrils from ME7, 79A and 22F mouse-passaged scrapie.

SAF were purified from the brains of mice infected with ME7, 22F or 79A strains of mouse-passaged scrapie. Duplicate preparations were made and the 6 samples were reduced and alkylated in 6 M guanidine hydrochloride prior to tryptic digestion. Tryptic peptides were separated and detected by online LC-MS/MS and the data searched against the IPI murine database by use of the Mascot search algorithm. Proteins are listed in the table if they were identified conclusively in at least one SAF preparation. Protein hits were deemed to be conclusively identified if they were above the level of significance (as determined by Mascot scores) and more than one peptide matched to the sequence. Proteins identified in these experiments but that had previously been identified in control preparations from uninfected brains are not listed in this table, but full compiled results are available (Table S1). nd – not detected. dbcl – protein detected but below the level of confidence because only a single peptide was matched.a– due to extensive sequence homology between different chains of these proteins it is not possible to tell whether only a single isoform or multiple isoforms are present.