Resolving the complete genome of Kuenenia stuttgartiensis from a membrane bioreactor enrichment using Single Molecule Real-Time sequencing

In this study, we present the first closed genome sequence of an anammox bacterium, Kuenenia stuttgartiensis MBR1. It was obtained through Single Molecule Real-Time (SMRT) sequencing of an enrichment culture constituting a mixture of highly similar Kuenenia strains. MBR1 is a novel strain that is different from the previously reported Kuenenia KUST reference genome. Numerous structural variations and unique genomic regions have been identified, indicating diversification between strains.