RNAseq data of two week-old soybean leaves response to cold stress

Transcriptome analysis of cold-treated leaves (unifoliates) of soybean seedlings were performed. RNAseq analysis was performed using two lanes on a Illumina HiSeq2000 and sequenced on a 100bp, paired-end run. Two-weeks old soybean (c.v. ‘Williams 82’) seedlings were cold-treated (4 °C) starting at 4 h after the lights turned on (Zeitgeber Time, ZT4 h, 18 hours light/6 hours dark) and maintaining 4 °C continuously with the light cycle till harvest time (0, 1, and 24 hours). All treatment samples were performed in triplicate (with n≥6 plants per replication).